Journal: Oncology Letters

Article Title: Osteopontin improves adhesion and migration of human primary renal cortical epithelial cells during wound healing

doi: 10.3892/ol.2016.5219

Figure Lengend Snippet: Effect of OPN knockdown or over-expression on adhesion protein and GAPDH expression in the human primary renal cortical endothelial cell line. (A) The expression of pY118-paxillin, pY-FAK, total paxillin and total FAK was examined by western blot analysis. (B) Statistical analysis of protein expression from western blotting. All data are presented as the mean ± standard error of the mean of 4 individual experiments. **P<0.01. OPN, osteopontin; FAK, focal adhesion kinase; over OPN, over-expression of pcDNA3.1-OPN-green fluorescent protein; over blank, empty carrier over-expression; si-OPN, small interfering RNA interference; si-blank, small interfering RNA.

Article Snippet: Antibodies against OPN (rabbit polyclonal anti-human; 1:1,500; ab8448), phosphorylated (p)-Y118-paxillin (rabbit polyclonal anti-human; 1:1,000; 2541), GAPDH [rabbit polyclonal anti-human; XP ® Rabbit mAb (HRP Conjugate); 1:2,000; 8884), paxillin (rabbit anti-human; 1:1,000; 2542), FAK (rabbit polyclonal anti-human; 1:1,000; 3285) and pY Tyr576/577-FAK (rabbit polyclonal anti-human; 1:1,000; 3281) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

Techniques: Knockdown, Over Expression, Expressing, Western Blot, Small Interfering RNA

Journal: Oncotarget

Article Title: Suppression of osteopontin inhibits chemically induced hepatic carcinogenesis by induction of apoptosis in mice

doi: 10.18632/oncotarget.13529

Figure Lengend Snippet: A . Representative photomicrographs of immunohistochemistry for EGFR in liver tissue samples of WT and OPN KO mice at 36 weeks after DEN injection. EGFR expression in the cellular membrane of hepatocytes was stronger in WT mice than in OPN KO mice. Bar = 400 μm (inserts) B . RT-PCR and western blot for EGFR and c-Jun. OPN suppression caused a decrease in EGFR transcription, accompanied by the downregulation of nuclear c-Jun expression in the Hep3B and Huh7. The results are presented as SEMs (n=4). * P < 0.05 versus control cells. C . Western blot for EGFR and ERK. OPN KD cells Hep3B and Huh7 showed lower expression levels of EGFR and phosphorylated ERK1. The results are presented as means ± SEMs (n=4). * P < 0.05 versus control Hep3B and Huh7. D . Western blot for EGFR and ERK according to supplementation with rhOPN in OPN KD Hep3B and Huh7. The expression of EGFR and phosphorylated ERK was increased by rhOPN treatment in a time-dependent manner. The results are presented as means ± SEMs (n=3 for each condition). * P < 0.05 versus OPN KD Hep3B and Huh7.

Article Snippet: Anti-OPN antibody (1:50; R&D Systems) or anti-EGFR antibody (1:100; Ventana Medical Systems, Oro Valley, AZ, USA) were employed as the primary antibody.

Techniques: Immunohistochemistry, Injection, Expressing, Membrane, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control

Journal: Oncotarget

Article Title: Suppression of osteopontin inhibits chemically induced hepatic carcinogenesis by induction of apoptosis in mice

doi: 10.18632/oncotarget.13529

Figure Lengend Snippet: A . Representative photomicrographs of IHC for OPN and EGFR. Serially sectioned human HCC samples showing strong (a, d), moderate (b, e) and weak expression of OPN and EGFR (c, f). Bar = 400 μm (inserts). B . Correlations among OPN expression, pTNM, stages and EGFR expression.

Article Snippet: Anti-OPN antibody (1:50; R&D Systems) or anti-EGFR antibody (1:100; Ventana Medical Systems, Oro Valley, AZ, USA) were employed as the primary antibody.

Techniques: Expressing